Biolife, Volume 3, Issue 2, 2015
DOI: http://dx.doi.org/10.17812/blj2015.32.4a


Research Article:






Dalal BOUDOUDOU1, Anas FADLI2,  Abdelhak TALHA3, Younes BOURACHDI4 , Allal DOUIRA5
and Hamid BENYAHIA6

1,2,3,5Laboratory of Plant Science, Biotechnology and Crop Protection, Department of Biology, Faculty of Sciences, Ibn Tofail University, BP. 242, Kenitra (Morocco)
4Abbes Kabbage group, Belkssiri, kenitra (Morocco)
6Unit of Plant Breeding and Germoplasm Conservation, laboratory of citrus rootstocks breeding, National Institut of Agricultural Research (INRA), Regional Center of Kenitra,  BP 14000, (Morocco)


*Email: hamidbenyahia2002@yahoo.fr


Abstract


Phytophthora root rot is considered as the most damaging disease for citrus production in Morocco. Phytophthora species are generally present as propagules in citrus fields colonizing the root zone of rootstocks and representing a source of infection for the aerial parts and a risk of dissemination to new fields. This study aimed to monitor the seasonal evolution of the inoculum density of Phytophthora spp. in heavy ground at the Gharb region in Morocco in the rhizospher of two rootstocks: Citrus aurantium L. and Citrus macrophylla. Soil samples were taken at monthly intervals over a period of 14 months between April 2013 and June 2014. The inoculum density of Phytophthora species was estimated using the dilution technique in conjunction with the selective BARPHY 72 medium. Phytophthora isolates were identified based on colonies’ morphological and taxonomic criteria as well as mycelial characteristics. In both plots, Phytophthora parasitica was the predominant isolated species, followed by P. citrophthora. The inoculum density of Phytophthora fluctuated throughout sampling period in spring and summer, depending on environmental conditions, and rootstocks.

Keywords
: Root rot, Phytophthora, seasonal evolution, P. parasitica, P. citrophthora.


Published online on  5th April, 2015
© 2015 Published by Global Science Publishing Group, USA


How to Cite this Article

Dalal BOUDOUDOU, Anas FADLI,  Abdelhak TALHA, Younes BOURACHDI , Allal DOUIRA and Hamid BENYAHIA (2015). Effect of seasonal and citrus rootstocks on inoculum density of Phytophthora spp. in citrus orchard in a heavy soil of the Gharb region of Morocco. Biolife, 3(2), pp 367-377. doi:10.17812/blj2015.32.4a
Link to this article: http://biolifejournal.com/10.17812_blj2015.32.4.html
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Effect of seasonal and citrus rootstocks on inoculum density of
Phytophthora spp. in Citrus orchard in a heavy soil of the Gharb region of
Morocco
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