Biolife, Volume 3, Issue 2, 2015
DOI: http://dx.doi.org/10.17812/blj2015.32.16

Research Article:




Divya Teja, D1 and VivekaVardhani, V2*

1Department of Biochemistry, Acharya Nagarjuna University, Nagarjunanagar-522 510, Andhra Pradesh. INDIA
2Department of Zoology & Aquaculture, Acharya Nagarjuna University, Nagarjunanagar-522 510, Andhra Pradesh. INDIA

*Email: divya.darvemula@gmail.com

Abstract

Total protein, DNA and RNA was estimated in the abdominal muscles of experimental and control male swiss albino mice (6-8 wks old; 23-26 g wt). Immunex DS (IDS) was orally administered (@150mg/mouse) as a single dose in mice of group I. In a set of 6 experimental groups, IDS was orally administered @150mg/mouse  in a single dose on day 0 and injected with Gene Vac B vaccine on day 4 of experiment (@ 0.07ml/mouse, A; 0.1ml/mouse, B; 0.2ml/mouse, C; 0.4ml/mouse, D; 0.8ml/mouse, E, and 1ml/mouse, F). A single group was kept as control (untreated and uninfected). Two mice from all the eight groups were sacrificed on day 8, 9, 10, 11 and 12 after vaccine treatment. Muscle tissue (abdominal) was separated and analysed for total protein, DNA and RNA using standard methods. The level of protein, DNA and RNA showed much alteration in all the experimental groups of mice throughout the infection period when compared with controls and IDS treated mice; this abnormality might be due to IDS and/or pathogenic stress caused by reaction oxygen species.

Key words: Protein, DNA, RNA, Abdominal muscles, mice, Immunostimulant, Hepatitis.


Published online 14th May, 2015
© 2015 Published by Global Science Publishing Group, USA


How to Cite this Article

Divya Teja, D and VivekaVardhani, V (2015). Total protein, DNA and RNA content in the abdominal muscles of mice treated with Immunex DS and Hepatitis B Vaccine. Biolife, 3(2), pp 469-475. doi:10.17812/blj2015.32.16


Link to this article: http://biolifejournal.com/10.17812_blj2015.32.16.html
Biolife
AN OFFICIAL PUBLICATION OF GLOBAL SCIENCE PUBLISHING GROUP, USA
International Quarterly Journal of Biology & Life Sciences
Impact
Factor

4.175
eISSN 2320-4257
Total protein, DNA and RNA content in the abdominal muscles of mice treated with Immunex DS and Hepatitis B Vaccine

  Article Options
Copyright © 2015 by http://globalsciencepg.org/ ·  All Rights reserved  ·  E-Mail: editorbiolife@gmail.com